597 papers
This study establishes a reproducible, ISSCR-compliant protocol for generating and characterizing rhesus macaque induced pluripotent stem cells (RhiPSCs) from MAPT R406W mutation carriers, providing a critical resource for cross-species in vitro modeling of genetic frontotemporal dementia.
This paper introduces single-cell APEX-seq, a proximity labeling-based method that maps subcellular transcriptomes at single-cell resolution to reveal interaction-dependent cell states and identify regulators like CTSW that enhance CAR T cell persistence and tumor killing, which are often missed by conventional scRNA-seq.
This study identifies a rare Tcf4 lineage-negative stem cell population in the colon that is essential for wound repair and serves as the specific cell of origin for Apc-deletion-induced tumors in a Tcf4-haploinsufficient background.
This study identifies calcium-responsive centrin-Sfi1 protein networks as the molecular basis for the ultrafast, ATP-independent contraction in the giant ciliate *Spirostomum ambiguum*, demonstrating how their fishnet geometry and calcium-triggered compaction enable rapid whole-cell shortening through a mechanism distinct from traditional actomyosin systems.
This study reveals that Inhibitor-2 acts as a central chaperone in a dynamic cycle that delivers PP1 catalytic subunits to regulatory adapters, a process critically dependent on its RVxF docking motif to couple the relief of active site inhibition with the successful handoff to adapter-bound holoenzymes.
This study demonstrates that acid stress reprograms oral epithelial cells toward pro-inflammatory and pro-oncogenic phenotypes by inducing morphological changes resembling epithelial-mesenchymal transition, altering the expression of metabolic and immune genes, and increasing TGF-β1 production in response to microbial ligand challenges.
This study utilizes a patient-specific iPSC-derived liver model to demonstrate that the drug SAHA reduces toxic Z-alpha-1-antitrypsin polymers by upregulating heat-shock protein chaperones, thereby mitigating ER stress in alpha-1-antitrypsin deficiency.
This study reveals that C9ORF72-derived polyGR polypeptides disrupt nucleocytoplasmic transport in ALS/FTD by non-linearly retuning the nuclear pore's selectivity barrier, causing a biphasic modulation where proteins with moderate hydrophobicity exhibit enhanced transport while highly hydrophobic proteins are suppressed and accumulate in the cytoplasm.
This study reveals that TRIM21 functions as a molecular rheostat for influenza A virus replication by exerting expression-dependent, graded effects that couple viral nucleoprotein ubiquitination with innate immune modulation, while simultaneously restraining a latent PRKDC-mediated antiviral pathway.
This study reveals that lipid droplet accumulation delays regulated cell death by actively sequestering pro-apoptotic regulators like Bax through enhanced lipid droplet-mitochondria contacts, thereby protecting cells from apoptosis and offering potential therapeutic targets for apoptosis-resistant cancers.
This study reveals that cardiomyocytes possess an intrinsic immunocompetent capacity where IFNγ-induced GBP5 drives a pro-inflammatory AIM2/CASP1 pathway while simultaneously engaging a non-canonical GBP5/TGFβ axis to exert anti-inflammatory feedback, thereby resolving the cytokine's paradoxical roles in cardiac inflammation.
This study demonstrates that SNED1 fibrillar assembly in the extracellular matrix depends on the presence of fibronectin and collagen I, with collagen I identified as the first direct binding partner of SNED1.
This study demonstrates that while SNED1's initial incorporation into the extracellular matrix is independent of integrin binding, its subsequent matrix assembly, structural patterning, and promotion of cell proliferation and alignment are specifically driven by interactions with LDV-binding integrins rather than RGD-binding integrins.
This paper presents a vision transformer-based classification framework that utilizes kymographs to more accurately predict CMT2A disease phenotypes in iPSC motor neurons compared to traditional summary statistics, thereby enabling scalable computational screening for mitochondrial trafficking defects.
This study identifies that the 53BP1-USP28-p53-mediated external mitotic surveillance (EMS) pathway acts as a fail-safe mechanism to induce post-mitotic arrest when internal fidelity-ensuring controls like the spindle assembly checkpoint are compromised, effectively validating mitotic outcomes from outside the process.
The paper introduces CycSTORM, an automated cyclic super-resolution imaging platform that integrates fluidic exchange, active drift correction, and rapid chemical inactivation to enable high-throughput, multi-day, nanoscale mapping of multiple protein targets within single cells with sub-5 nm registration precision.
This study reveals that the leukemogenic fusion protein NUP98-KDM5A forms gel-like condensates preferentially at genomic loci with high local H3K4me3 density, a quantitative targeting mechanism that drives specific activation of HOX genes and underlies the disease's transcriptional program.
This study reveals that oxidative stress triggers a conserved, dynein-dependent mechanism for clustering multiple membrane-bound organelles around the nucleus via a non-canonical pathway, which in turn potentiates adaptive gene expression through the coordinated spatial positioning of these compartments.
This study reveals that Syntaxin11 deficiency causes familial hemophagocytic lymphohistiocytosis 4 by impairing the priming and multimeric assembly of Orai1 channels, which subsequently inhibits calcium entry, NFAT activation, and critical T-cell functions like cytotoxicity and cytokine production.
This study reveals that human hematopoietic stem and progenitor cells inherit a "clonal memory" of division synchronicity and fate commitment across multiple generations, a regulatory mechanism that is disrupted in acute myeloid leukemia but can be partially restored through epigenetic modulation.